Urinary single-cell sequence analyzes the urinary macrophage in different outcomes of membranous nephropathy

Abstract Background Great progresses have been made in diagnosis and treatment of membranous nephropathy (MN). However, a significant number patients do not respond to immunosuppressive therapy eventually progress ESRD. To investigate the mechanism different outcome MN, we performed single-cell sequencing analysis urine cells with without complete remission MN. Methods Urine RNA was on 12 healthy controls (HC) 15 The were divided into group (CR, n = 9) no (NR, 6). Results 1) Macrophages largest cells, they 48.02%, 68.96% 20.95% HC, CR NR group, respectively. 2) Urinary macrophages expressing FIColin-1 S100 calcium binding protein A8 mainly HC indicating that derived from bone marrow peripheral blood, while urinary regulator G-protein signaling 1 HLA-DPA1, renal resident macrophages. 3) In adults, expressed metallothionein family, can regulate anti-inflammatory proinflammatory functions bidirectionally. showed strong properties. associated level proteinuria impaired function. Conclusions Our study firstly delineated differences cell maps between individuals MN or outcomes. Not only origin but also function group.